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(D) ROS production (E), MDA content (F) and SOD content (G) in cells were measured by 2’-7’-Dichlorodihydrofluorescein diacetate assay or ELISA. 2’-7’-Dichlorodihydrofluorescein diacetate assay was used to detect reactive oxygen species (ROS) production in testis tissue of rats (A). Then, [https://wopid.io/@kendraangelo83?page=about](https://wopid.io/@kendraangelo83?page=about) the culture medium was discarded, and the cells were made into a single cell suspension with a density of 1×106 cells/mL. Flow cytometry with Annexin V/propidium iodide (PI) staining was used to measure the apoptosis of Leydig cells. After transfection for 24 hours, we harvested the cells and analyzed them with a dual luciferase reporter kit (Promega, Madison, Wisconsin, USA) to standardize luciferase reporter activity to Renilla luciferase activity. Next, [https://git.warze.org](https://git.warze.org/aidanbooze5410) the HSD17B7 3’UTRs or HSD17B7 3’UTRs-WT and miR p mimic or a corresponding negative control (NC mimic) were co-transfected into HEK-293T cells with Lipofectamine™ 3000 (Invitrogen, Carlsbad, CA, USA) when grown to approximately 70% confluence. 2’-7’-Dichlorodihydrofluorescein diacetate was used to detect the production of ROS in cells and tissues. Finally, they were put into an enhanced chemiluminescence system (Bestbio, Shanghai, China) to develop the signals and scan the protein bands with Image J software. As an effective antioxidant, it has many beneficial effects, such as anti-cancer , anti-inflammatory, antioxidant , and hypoglycemic properties . The relative weights of the testis, epididymis, seminal vesicles, and prostate of diabetic rats were significantly reduced. 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We demonstrated that TQ mitigated BPH in rats and showed no toxicity to the liver and reproductive system. In the current study, we explored the anti-BPH effects of TQ in vivo and identified its main therapeutic component and the underlying mechanisms in vitro. Existing pharmacotherapy shows several side effects, and the exploration of new therapeutic strategies is of high significance. Return to the main supplement interaction checker page Other laboratory research also shows that quercetin inhibits CYP3A4. Theoretically, concomitant use might alter the effects and adverse effects of CYP3A4 substrates. The same amount of proteins (20 µg) was packed into each electrophoresis tank, then separated by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and transferred to polyvinylidene fluoride membranes (Millipore, Billerica, MA). The total protein concentrations were detected with bicinchoninic acid protein assay kit (Beyotime, Shanghai, China). The TRIzol reagent (Invitrogen, Carlsbad, CA, USA) was used to extract total RNA from cells and tissues, according to the manufacturer’s instructions.